VALIDATED RP-HPLC METHOD FOR THE ESTIMATION OF TELMISARTAN IN TABLET FORMULATION

A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method is developed and validated for the estimation of telmisartan in tablet dosage form. The expected separation and peak shapes were obtained on chromosil C18 (250 mm x 4.6 mm, 5 μm) column. To have an ideal separation of the drug under isocratic conditions, mixtures of solvents like methanol, water and acetonitrile with or without different buffers in different combinations were tested as mobile phases on a chromosil C18 column. A mixture of methanol : 0.1% orthophosphoric acid : acetonitrile in the ratio of 80:05:15 v/v/v was proved to be the most suitable of all the combinations since the chromatographic peak obtained was better defined and resolved and was almost free from tailing. The flow rate was 1.5 ml/min and effluents were monitored at 256 nm. The retention time for telmisartan was 2.7 min. The method was validated and found to be accurate, and precise. Recovery of telmisartan from tablet formulation was found to be 99.41%. The proposed method was successfully applied for the quantitative determination of telmisartan in tablet formulation.