Prevalence of Bovine Viral Diarrhea virus in West Bengal, India

Bovine Viral Diarrhea (BVD) is one of the most economically important diseases in cattle. The present study was undertaken to diagnose the persistently infected (PI) animals by AntigenELISA and Reverse Transcriptase PCR using serum samples from organized farms as well as rural areas of West Bengal. The results showed that out of 964 serum samples tested 07 (0.73%) was positive for BVDV by Antigen-ELISA. For further confirmation, RNA was extracted from the positive samples and RT-PCR was performed with 5' UTR specific primers which showed 294 bp amplicons. This finding showed circulation of BVDV in cattle in West Bengal, India.