COMPARATIVE STUDIES OF NANOPARTICLES AND MICROSPHERES OF MINOCYCLINE HYDROCHLORIDE AS LOCAL DELIVERY IN TREATMENT OF PERIODONTITIS

Periodontitis is an inflammatory disease of the supporting tissues of the teeth caused by groups of specific microorganisms. Local delivery of tetracycline antibiotics has been investigated for the possibility of overcoming the limitations of conventional therapy. The goal of this research work is to prepare nanoparticles and microspheres containing minocycline hydrochloride, these tiny particles are formulated into in-situ gels as a local drug delivery system within the periodontal pockets for the effective treatment of periodontitis. FT-IR studies indicated that there was no chemical interaction between drug and polymer and stability of the drug. The microspheres were prepared by emulsion cross linking method using chitosan as a polymer. Nanoparticles containing Minocycline hydrochloride were developed by using eudragit RL 100 as polymer. Microspheres and nanoparticles were evaluated for drug content, particle size analysis and stability studies. The physicochemical parameters reveal that microspheres have a discrete spherical structure without aggregation. The average particle size of minocycline nanoparticles and microspheres were within the range. On the other hand optimized formulations ofin-situ gels containing nanoparticles, microspheres and pure drug were prepared by using gellan gum (0.6 % w/v) and comparative in-vitro diffusion study have done for these in-situ gel formulations. No appreciable difference was observed in the extent of degradation of product during 60 days in which nanoparticles and microspheres were stored at 40°C/ 75% RH. Key words: periodontitis,minocycline hydrochloride, nanoparticles, microspheres and In-situ gels