IMMOBILIZATION OF THIAMINE KINASE FROM PIG LIVER BY THE MICROENCAPSULATION METHOD

The article investigates immobilization of thiamine kinase (EC 2.7.6.2) from pig liver by microencapsulation into semipermeable membranes, protein behavior and its stability in the designated structures. At the same time enzyme lyophilization by the sublimation method was performed. It was shown that microencapsulated protein operated in the kinetic-diffusion regime, which influenced the way of kinetic curves passage and values of the obtained effective constants. When the enzyme was included in hydrophobic microcapsules made of butadiene latex the deviations of constants stayed within acceptable calculated values; when included in hydrophilic microcapsules on cellulose nitrate base ? the values were much more significant. The immobilization of thiamine kinase increased its stability and thermolability. First of all this was correct for enzymatic preparations incorporated in hydrophobic carrier. Hydrophylic interactions were not so effective. The highest stabilization effect was obtained with thiamine kinase lyophilization.