A VALIDATED RP-HPLC METHOD FOR ESTIMATION OF GUAIFENESIN IN BULK DOSAGE FORM
Journal: Indo American Journal of Pharmaceutical Sciences (IAJPS) (Vol.3, No. 1)Publication Date: 2016-02-14
Authors : Hajare Pranit Pandurang; Laware Ravindra Bhimraj; Bhusal Ramesh Dattatraya; Magar Manisha Mhasu;
Page : 44-51
Keywords : Guaifenesin; Methanol; Development; RP-HPLC; Validation;
Abstract
A simple and reproducible method was developed for Guaifenesin by Reverse Phase High Performance Liquid Chromatography (RP-HPLC). Guaifenesinwas separated on Greece C18 (4.6ID x 250mm, Particle size: 5 micron), using ortho phosphoric acid buffer with pH of 3.0 at the UV detection of 225 nm. Isocratic elution of methanol and water was used as a mobile phase with various ratios and flow rates, eventually 80:20 v/v methanol and water was being set with the flow rate of 0.8mL/min. The statistical validation parameters such as linearity, accuracy, precision, LOD, LOQ, Robustness, system suitability were checked, further the limit of detection and limit of quantification of Guaifenesin concentrations were found to be 57.98?g/mL and 175.71?g/mL . Keywords:Guaifenesin, Methanol, Development, RP-HPLC, Validation
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