BRUCELLINA PPD PRODUCTION AND MONITORING OF VACCINATED CATTLE WITH VACCINE WITH STRAIN BRUCELLA ABORTUS RB51

Brucella infection is present in Albania, as in small ruminants, also in cattle. Prevalence of Brucella infection in small ruminants is at 0.9 to 1.2%, whereas this infection in cattle has endemic character and is identified in some areas of the country, as in Korce, Durres, Diber, Lushnja and Gjirokastra. The strategy of control / eradication of brucellosis in cattle in some complexes, along with elements of serological control and the elimination of positive heads of cattle, was applied the immunization of cattle with the vaccine produced by vaccine strains Brucella abortus RB51. To identify cattle vaccinated and those infected with Brucella spp, was produced Brucellina PPD allergen. For the production of Brucellina PDP allergen was used strains Brucella abortus RB 51. Bacterial emulsion produced, underwent several rigorous methodological procedures, like: emulsion deactivation in biofermentator in temperature 700 C, centrifugation at 10.000 and 15.000 g/min, separation of the supernatant, the benefit of cytoplasmic protein by processing it with ATC and purification through the use of tampons solutions with pH 11, pH 9.6, pH 2.6 and pH 7.0 - 7.2. After these procedures through Kjeldhal method was enabled determine the concentration of the purified cytoplasm protein. The product received was finalized at that level,at which 1 ml product contained 1μg protein. The study has been extended in 1250 head of cattle from complexes of Tirana, Durres and Lushnja, conducted during the years 2009 to 2012. With the applied strategy, it was achieved that after 18 months, the complex of cattle in Durres was considered practically healthy, while the cattle complex in Lushnja is on the eve of health. Sensitivity of allergy test in vaccinated cattle resulted 94%, while the sensitivity of this test in a limited number of heads of cattle, considered positive with serological tests, resulted 81.25%, versus specificity which is 100%. This study shows that Brucellina PPD allergen produced with strains Brucella abortus RB 51 can be used to identify vaccinated cattle from the infected cattle by using Brucella spp. ATC-Acid trichoracetik.