GENETIC DIVERSITY OF TWO IMPORTANT GENES FROM SUCKING AGRICULTURAL PEST APHIS GOSSYPII (G) POPULATIONS COLLECTED FROM KARNATAKA, INDIA

Genetic diversity of two important genes (sodium channel and USP) from intra specific populations of Aphis gossypii were carried out for a better understanding of sequence variations, which facilitates enumeration of multi-host versatilities in Aphis gossypii. Thus, upon cloning, sequencing and aligning, 23 out of 586 bp (3.92%) and 24 out of 1146bp (2.09%) nucleotide variations were chronicled for sodium channel and USP genes respectively with 2 parsimony informative sites for both the genes. Further, our study concluded that there was a narrow sequence variation observed between populations on constructing a phylogenetic tree which resulted in a single clade for both the genes. The information generated from the present study aids in future insect pest management strategy like RNA interference which is futuristic, safe and acts as sequence specific biopesticide.