Multiple shoot production from nodal explant and FTIR analysis of in vitro regenerated plants of Psoralea corylifolia L.

Objective: Multiple shoot induction was achieved from nodal explants of Psoralea corylifolia with cytokinins. FTIR analysis was done to know the functional groups present in the leaf extract.Methods: MSB5 medium supplemented with BAP, KIN and GA3 at different concentrations were used to get multiple shoots and their elongation from nodal explants. Rooting of the elongated shoots was done on medium supplemented with different concentrations of auxins. Leaves were collected from in vitro regenerated plants, extracted using petroleum ether and subjected to FTIR analysis.Results: In present study, high frequency of multiple shoot induction was achieved using nodal explants of P. corylifolia with BAP and KIN. FTIR analysis revealed the presence of different functional groups present in the leaf extract obtained from in vitro regenerated plants.Conclusions: P. corylifolia is one of the important medicinal plants which has numerous valuable secondary metabolites. These compounds are used for treating various diseases caused by several clinical pathogens.