Development and validation of first order derivative spectroscopy method for simultaneous estimation of candesartan cilexetil and pioglitazone hydrochloride in synthetic mixture

Objective: Objective of the study was to develop a simple, accurate, precise, economic, robust and rugged UV spectrophotometric method and validate for the simultaneous estimation of Candesartan Cilexetil and Pioglitazone Hydrochloride in synthetic mixture.Methods: Combination of Candesartan Cilexetil and Pioglitazone Hydrochloride has only one LC-MS/MS analytical method developed yet, so this UV method was novel for combined synthetic dosage of Candesartan Cilexetil and Pioglitazone Hydrochloride (1:2). This method utilizes methanol as a solvent. Zero crossing point (ZCP) of Candesartan Cilexetil and Pioglitazone hydrochloride were found to be 242.00 nm and 327.60 nm respectively. Hence, estimation of Candesartan Cilexetil and Pioglitazone Hydrochloride were done at 327.60 nm and 242.00 nm respectively.Results: The linearity of Candesartan Cilexetil and Pioglitazone Hydrochloride were found to be in range 10-50 μg/ml and 20-100 μg/ml (R2=0.9995) respectively. The accuracy of the present method was evaluated at 80%, 100% and 120%. Recovery was found to be in range 99.50-100.80% and 99.27%-101.36% for Candesartan Cilexetil and Pioglitazone Hydrochloride respectively. Intermediate precision studies were carried out and the RSD values were less than one. The value of LOD and LOQ were found to be 0.170 and 0.516 μg/ml for Candesartan Cilexetil and 0.207 and 0.625 μg/ml for Pioglitazone Hydrochloride.Conclusions: It can be concluded from the results that the proposed method was sensitive, accurate, precise and reproducible for the simultaneous determination of Candesartan Cilexetil and Pioglitazone Hydrochloride in Synthetic Mixture.