Validation of Fiber Quality Linked SSR Markers Derived from Allotetraploid (Gossypium hirsutum) in Diploid (Gossypium arboreum)

Abstract. Present study was conducted to estimate genetic diversity for fiber quality traits among elite G. arboreum and G. hirsutum cotton genotypes using simple sequence repeat (SSR) markers; and to establish relationship between genetic diversity of all three species including 13 genotypes. The experimental plant material included 13 genotypes viz., 824, CINA 329, CINA318, Jawahar Tapti, CINA344, CINA333, DLSA17, G. cot H12, G. cot H4, G. cot H16, G. cot H10, G cot 8 and G. cot 20. The genotypes were amplified by 29 SSR primer pairs belonging to BNL (11), CIR (06), NAU (08) and JESPR (04) series. All 29 primers showed amplification and produced a total of 343 bands. Average number of bands amplified by each primer was 11. Statistical analysis for SSR data was conducted using software program me NTSYS pc version 2.02e. The genetic distance (GD) among the cotton genotypes ranged from 0.19 to 0.70. The highest GD (0.70) was detected between 824 and CINA344 while the lowest GD (0.19) was observed between G.cot H10 and G.cot 20. The average observed mean heterozygosity was 0.70 and observed mean percentage of polymorphic loci was 70%.