Studies on biosynthesis of auxin in rhizobium and their impact on growth of Vigna mungo L.

Objective: The present investigation, the use of plant growth promoting rhizobacteria was used to growth and improves the yield in crop of Vigna mungo.Methods: Rhizobium strains were isolate and identified by Yeast Extract Mannitol Agar medium with congored (CYEMA) and some biochemical test were performed. Separation and quantification of growth hormone by paper chromatography and spectrophotometric methods followed and its impact of Vigna mungo in green house experiments.Results: The Rhizobium strains were isolated from legumes plant Mimosa pudica L, Arachis hpogea L and Vigna mungo L. Indole-3-acetic acid (IAA) produced from the L-trptophan medium and identified by using paper chromatography techniques. The pot culture experiments were conducted with various treatment of Rhizobium sp, strains are high growth and yield.Conclusions: Rhizobium is beneficial organisms and to interaction with growth promotion to all leguminous plants as well as nitrogen fixation, its biological process of the ecosystem. It is concluded to the Rhizobium species is eco-friendly to farmer and these environments.