Simple and Flexible Microfabrication of Optical Biosensing Films Using Electrophoretic Deposition

We propose a simple approach for the development of optical glucose sensing membranes adaptable to micro and nanofabrication technologies. The glucose sensitivity derives from the use of the enzyme glucose oxidase (GOx) and oxygen sensing polymeric particles. These two elements have been integrated onto gold coated chips in alternating layers using electrophoretic deposition (EPD). The oxygen sensing particles were obtained by precipitation-evaporation after covalently linking poly (styrene-co-maleic anhydride) and oxygen sensing Pt (II) porphyrin using click chemistry. The membrane is capable of determining glucose between 0.07mM and 3mM with a limit of detection of 0.02mM. The ability of EPD to adapt to substrates with different shapes and size allows its further implementation into different types of microdevices.