“Zero-Crossing” First-order Derivative Spectroscopy Method for Analysis of Sertraline Hydrochloride in Novel Self Micro Emulsifying Drug Delivery System

Novel Self-Micro Emulsifying Drug Delivery System (SMEDDS) of Sertraline hydrochloride was developed. SMEDD System of Sertraline hydrochloride consists of Capmul MCM C8 EP as oily phase, Cremophore RH 40 as surfactant and Labrafil M 2125 as co surfactant. These self-emulsifying additives interfere significantly with the estimation of Sertraline hydrochloride by UV spectrophotometry method. In the present study “Zero crossing” first-order derivative spectroscopy method was developed and validated to estimate the Sertraline hydrochloride in presence of self emulsifying additives and compared with simple UV-Visible spectrophotometry method. The obtained results noticeably recommended that, in comparison with the UV-spectrophotometry method; the first-order derivative UV-spectrophotometry is a simple method to estimate Sertraline hydrochloride with sufficient accuracy, specificity and precision. The proposed methods were applied successfully to estimate the drug content and in vitro drug release of newly formulated Self emulsifying drug delivery system of Sertraline hydrochloride.