Influence of Various Parameters on Cellulase and Xylanase Production by Different Strains of Trichoderma Species

The main purpose of this study is to reduce the production cost of cellulase by using alternative carbon source such as wheat bran residue and optimized fermentation medium for high yields. In the present investigation, to isolate the novel cellulase producing fungi, Trichoderma viride and T. reesei from the soil samples and to optimize the physicochemical and nutritional parameters for cellulase and xylanase production. Wheat bran residue was found to be the best substrate source for the production of cellulase and xylanase by Trichoderma spp. Optimal concentration of wheat bran residue for the production of cellulase and xylanase was 1.0% (w/v). Optimum temperature, pH and incubation time of the medium for the cellulase and xylanase production by T. viride and T.reesei was 28° C, 5.5 and 120 h respectively. Cellulase production from T. reesei can be an advantage as the enzyme production rate is normally higher as compared to other fungi.