Lipopolysaccharide Responsive Beige-Like Anchor Subcellular Localization Involving in Vesicle Trafficking Responsive to Lipopolysaccharide

Lipopolysaccharide (LPS) responsive Beige-like Anchor (LRBA) gene is an important novel immune regulator, mutations of which cause common variable immunodeficiency, autoimmunity and inflammation. The underlying subcellular mechanism is unknown. Studies on LRBA subcellular localization and involvement in the dynamic vesicle trafficking may help to decipher its function of causing immune disorders. The present immune fluorescence confocal microscopy results show that LRBA is co-localized with Golgi proteins (GM-130, P-230 and GS-28), early endosome proteins [Early Endosome Antigen 1(EEA1), CLATHRIN, RAB4 and ADAPTIN-β], PKA subunits (RIIα, RIIβ and RIIC), and the microtubule protein, tubulin. Time lapse videos of live cells show LRBA-positive vesicles respond to LPS, which also stimulates LRBA nucleic translocation. This study demonstrates that LRBA is associated with the Golgi complex, endosomes, plasma membrane, nucleus, pseudopodia and microtubules, and vesicles. It suggests that LRBA plays anessential role in vesicle trafficking and signal transduction essential for normal immune response, especially against LPS-containing bacteria.