Simultaneous Estimation of Nebivolol and Amlodipine by UV Spectrophotometric Method
Journal: Current Trends in Technology and Sciences (Vol.1, No. 2)Publication Date: 2012-09-25
Authors : A. Gajbhiye N. Dwivedi;
Page : 118-121
Keywords : amlodipine; auxophores; chromophores; nebivolol; uv-vis spectrophotometry;
Abstract
Nebistar-SA is available for the treatment of stage II hypertension. It contains nebivolol (NV; 5 mg) and amlodipine (AM; 2.5 mg). In the present study, simple, rapid, precise and accurate method for the simultaneous estimation of these drugs have been developed and validated by UV spectrophotometry. The method was validated with respect to its linearity, limit of quantitation (LOQ), limit of detection (LOD), precision and accuracy. In this method, the NV and AM were scanned using methanol as solvent and λmax were found to be 218 nm and 237 nm for NV and AM respectively. For NV (A1 = 0.0182 Cx + 0.0409 Cy) and AM (A2 = 0.0016 Cx + 0.0034 Cy), the equations were developed by Vierodt’s method. LOD was found to be 0.086 ?g/ml for NV and 0.021 ?g/ml for AM. LOQ was 0.262 ?g/ml for NV and 0.064 ?g/ml for AM. The % RSD for day to day precision was 0.5316 for NV and 0.0056 for AM. The linearity was found to be in the range of 5-50 ?g/ml for NV and AM.
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