Development of quantitative method for analysis of Meropenem and Amoxycillin by RP-HPLC method

Objective: Development of quantitative method for analysis of Meropenem and Amoxycillin by RP-HPLC method.Methods: A simple, precise and accurate reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for the simultaneous determination of Meropenem and Amoxicillin. The chromatographic separation was performed on Cromosil C18 column (250 mm × 4.6 mm i.d, 5 μm particle size). Mobile phase consist of a mixture of phosphate buffer (pH 6.8) and acetonitrile in the ratio of 70: 30 v/v at a flow rate of 1.0 ml/min. The detection wavelength was set at 229 nm. The proposed method was validated for linearity, accuracy, precision, robustness, LOD and LOQ.Results: The calibration was linear over the range of 10-50 μg/ml for Meropenem and 10-50 μg/ml for Amoxicillin. The average retention time for Meropenem was found to be 5.41±0.015 min and Amoxicillin was found to be 2.73±0.03.Conclusions: The method can be easily adopted for quality control analysis.