EVALUATION OF POLYSTYRENE PETRI DISH - BASED METHOD FOR ASSESSING BIOFILM FORMATION IN VITRO BY C.GLABRATA AND ITS COMPARISON WITH TEST - TUBE METHOD

Journal: Indian Journal of Medical Research and Pharmaceutical Sciences (Vol.3, No. 9)

Publication Date: 2016-09-30

Authors : Singh Shewta; Hussain Nusrat; Bhattacharyya Sayan; Sarfraz Asim; Sengupta Abhishek; Kumar Dhirendra; A njum Nahid;

Page : 37-42

Keywords : Candida glabrata; Peptone water with dextrose; Safranine; Normal saline; Polystyrene petri dish; Test tube;

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Abstract

Introduction: - Biofilms formation plays an important role in fungal pathogenesis . Biofilms have been considered a virulence factor contributing to the Candidal infection. Therefore, a reliable method for their diagnosis is necessary. Materials and methods : In this study, biofilm formation of 10 isolates of Candida glabrata by Test tube method and polystyrene petri dish method was compared. Results: Petri dish method was found comparable with test tube method for studying biofim formation with better sensit ivity but poorer specificity. Conclusion: - Petri dish method can be safely used to find out pattern of biofilm formation by Candida glabrata.

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EVALUATION OF POLYSTYRENE PETRI DISH - BASED METHOD FOR ASSESSING BIOFILM FORMATION IN VITRO BY C.GLABRATA AND ITS COMPARISON WITH TEST - TUBE METHOD

Abstract

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