DESIGN AND CREATION OF VAGINAL LACTIC ACID BACTERIA ASSOCIATION RESISTANT TO PHYSIOLOGICAL CYCLIC PH DISCHARGES

Aim: The aim of this study is creation of sustainable consortium of symbiotic probiotic bacteria with high colonization potential for rapid response to vaginal acute and chronic challenges. Material and method: In this study used biocompatible lactobacilli and cocci possessing high antimicrobial activity, isolated previously from vaginal samples of healthy women from 20 to 45 years old from Armenia. The following methods: screening of antimicrobial activity, microbial adhesion to solvents, auto aggregation and co aggregation assays were used Statistical analysis used: Student's test computer, taking the criterion of P <0.05 sufficient for significant differences in the results. Results: The symbiotic consortium of Lactobacillus acidophilus GH 201 and Lactococcus lactis GH 204 strains shows higher inhibitory activities against bacterial pathogens and Candida albicans, intensive growth rate and biomass accumulation, than the single cultures The consortium is sustainable during multiple sub culturing imitating up and down changes of vaginal pH. Conclusion: The sustainable consortium of symbiotic probiotic bacteria have high colonization potential for rapid response to vaginal cyclic pH discharges caused by menses and frequently intercourses. These bacteria possess enhances growth rate, antimicrobial and adhesive effects, when working together