EFFECTS OF TUMOR ON CLUSTERING OF WATER IN PARTIALLY DEHYDRATED TISSUE OF RAT LIVER GUERIN CARCINOMA

The aim was to study the changes in the structure of intracellular water in the intact rat liver and liver with Guerin carcinoma. The state of water in lyophilized liver tissue of rats ? intact and affected by Guerin carcinoma at different stages of tumor development was studied by low-temperature 1H NMR spectroscopy. It is shown that in progress of tumor the contribution from the water clusters whose size is 3?12 nm is grows. It was established that in intact tissue present 6 types of water clusters capable of dissolving different amounts of trifluoroacetic acid. In the liver with Guerin carcinoma clusters of strongly associated water more uniform, and their dissolving power with respect to trifluoroacetic acid and the electron donor molecules significantly decreased. These results are important for elucidating the mechanism of action of water on the development of neoplastic processes.