MULTIPLEX POLYMERASE CHAIN REACTION FOR GENOTYPING OF Arabidopsis thaliana ECOTYPES USING SSLP MARKERS
Journal: Biotechnologia Acta (Vol.7, No. 4)Publication Date: 2014-08-30
Authors : Zimina O. V.; Sytnyk K. S.; Parii M. F.; Alkhimova O. G.;
Page : 80-84
Keywords : Arabidopsis thaliana ecotypes; SSLP markers; multiplex PCR.;
Abstract
The goal of the work was, using the database ?The Arabidopsis Information Resource? TAIR, to select 12 SSLP-markers distributed along the Arabidopsis chromosomes and chromosome arms, to optimize the conditions of amplification of each fragment and for simultaneous amplification of several fragments. For identification of A. thaliana ecotypes and their hybrid, the SSLP sequences were used. These DNA markers are highly polymorphic in Arabidopsis and easy to use. Using this database, the primers were selected for 12 SSLP-markers distributed along all chromosomes and their arms. A. thaliana ecotypes Columbia and Landsberg erecta were used. The experiments revealed that two-stage PCR using two annealing temperatures of primers in each cycle allows efficient amplification of all the fragments considered. The conditions for carrying out two multiplex PCR, each of which allows the two fragments were amplified and a single multiplex PCR allowing three markers for amplification were defined. The developed system of DNA markers can be used to study the behavior and inheritance of each chromosome of maternal and paternal genomes of Arabidopsis hybrids and enables quick and efficient genetic analysis.
Other Latest Articles
- IN VITRO PROPERTIES OF NEURAL CREST-DERIVED MULTIPOTENT STEM CELLS FROM A BULGE REGION OF WHISKER FOLLICLE
- HEMATOPOIETIC PROGENITOR CELLS OF PLACENTAL AND UMBILICAL CORD BLOOD: IMMUNOPHENOTYPIC ANALYSIS AND DIFFERENTIATION POTENTIAL in vitro
- NEW STRAINS PRODUCER OF BIOBUTANOL
- ISOLATION OF Phaffia rhodozyma YEASTS MUTANTS UNDER INCREASED CAROTENOID CONTENT
- Penicillium sp. ? PRODUCER OF EXTRACELLULAR α-L-RHAMNOSIDASE
Last modified: 2016-11-13 23:30:27