Sophora interrupta Bedd leafs aqueous extract inhibits proliferation of MCF-7 breast cancer cells by inducing apoptosis

The identification of medicinal plants and derived natural products for cancer therapeutics was long term developing goal. Here we investigated the anti proliferative and anticancer properties of Sophora interrupta Bedd aqueous extract on MCF-7 breast cancer cells. The cytotoxicity studies were performed by MTT and LDH assays reveals that its increase in dose dependant manner form 100-1000 ?g/ml for 24 hours. The microscopic studies revealed the morphological characteristics of apoptosis such as cell shrinkage, and membrane bubbling. The type of cell death was explored by fluorescence microscopy using the acridine orange/ethidium bromide (AO/EtBr) method. Significant number of treated cells because of late apoptosis was converted from green to orange color. The nucleus morphological features under gone apoptosis induced by the aqueous extract were evaluated by Hoechst 33258 staining, nucleus trails and apoptotic bodies increasing with increase concentration of aqueous extract. The dead cell count done by trypan blue method. Minimal or no oligonucleosomal DNA fragmentation was detected in untreated control cells. A clear dose response laddering pattern can be observed in treated cells. The morphological changes of cells well correlated with inter-nucleosomal DNA fragmentation. The present studies explain that aqueous extract of Sophora interrupta Bedd inducing the cell death through apoptosis and this may be potent drug target for mammary cancers. However further studies need to evaluate mode of action and molecular mechanism of Sophora interrupta Bedd inducing the cytotoxicity.