Sensitive Analytical Method Development and Validation of Ritonavir Bulk drugs by RP-HPLC

This present study report, a simple, specific, accurate and validated method using reverse phase-high pressure liquid chromatography for identification of Ritonavir. The method employs RP-HPLC (Younglin with UV/Visible detector), Inertsil Extend C18 (250 × 4.6 mm, packed with 5 ?m) and mobile phase comprises of A, B and C of Acetonitrile, methanol and buffer pH (6.2) at flow rate of 1.5ml/min and UV-detection at 256 nm. The method was validated in terms of system suitability, linearity and range, precision & accuracy study. The method was linear over the concentration range of 5-150?g/ml. the method showed good recoveries (98.7-101.9%). The method showed percentage relative standard deviation was found to be less than 2 and the method is specific for the identification for Ritonavir.