Estimation of Carbamazepine with Different Forms of Cyclodextrins in Pharmaceutical Preparation by RP-HPLC

A new simple, precise, rapid and selective reversed-phase high performance liquid chromatography (HPLC) method has been described for the determination of assay of carbamazepine (CBZ) with different forms of cyclodextrins in 1:1 mole ratio. The HPLC method depends on the separation of components of binary mixtures using Zorbax C18 coloumn with mobile phase consisting of water and methanol (40:60% v/v) with detection at 285 nm. A part from the simple blends analysis, and also the microwave mixtures were analyzed for CBZ content. The HPLC assay of CBZ was found to be 98 ? 102% in all the binary mixtures of alpha, beta, gamma and HP beta cyclodextrins. The accuracy and precision of the method has been determined and they have been validated by with pure drug analysis.