Probiotic Conjugated linoleic acid inhibits COX-2 Inflammatory pathway

Aim: Conjugate linoleic acid (CLA), a novel functional lipid , synthesised from linoleic acid by probiotic lactobacilli. In the present study, the CLA derived from Lactobacillus plantarum (LP-CLA) was assessed for its activity against COX-2 inflammatory pathway. Methodology & Results: The anti-inflammatory activity of Lactobacillus plantarum and LP-CLA was studied using chronic inflammation model .The in vivo experimental data was validated with in-silico analysis by docking CLA isomers into the proteins of inflammatory cascade using CHARM m-based molecular docking algorithm. The CLA isomers demonstrated stronger interaction than standard NSAIDs such as indomethacin and diclofenac .The impact of LP-CLA on the expression of COX-2 was investigated in MDA-MB-231 cells by Western blot analysis. The concentration-dependent inhibition of COX-2 expression was found without affecting the constitutive COX-1 protein expression in LP-CLA treated cells. Conclusion: The results demonstrated the inhibition of inflammatory cascade by conjugated linoleic acid derived from Lactobacillus plantarum.