STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF CEFOPERAZONE AND TAZOBACTAM BY USING RP-HPLC

A simple, Accurate, precise method was developed for the simultaneous estimation of the Cefoperazone and Tazobactam in Tablet dosage form. Chromatogram was run through Kromasil C18 250 x 4.6 mm, 5. Mobile phase containing Buffer Perchloric acid: Acetonitrile taken in the ratio 55:45 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% Perchloric acid buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 230 nm. Retention time of Cefoperazone and Tazobactam were found to be 3.751 min and 2.568min. %RSD of the Cefoperazone and Tazobactam were and found to be 0.4% and 0.5% respectively. %Recovery was obtained as98.77% and 98.58% for Cefoperazone and Tazobactam respectively. LOD, LOQ values obtained from regression equations of Cefoperazone and Tazobactam were 0.10, 0.31 and 0.02, 0.05 respectively. Regression equation of Cefoperazone is y = 7979.x + 15373, and y = 19820x + 5778 of Tazobactam. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries. Key Words: Cefoperazone, Tazobactam, RP-HPLC, Simultaneous estimation, Validation as per ICH guidelines, Stability studies.