STABILITY INDICATING RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF MUPIROCIN AND FLUTICASONE

A simple, Accurate, precise method was developed for the simultaneous estimation of the Mupirocin and Fluticasone in ointment dosage form by reverse phase high performance liquid chromatography. Chromatogram was run through Standard Discovery 250 x 4.6 mm, 5. Mobile phase containing Buffer Ortho phosphoric acid: Acetonitrile taken in the ratio 55:45 was pumped through column at a flow rate of 1ml/min. Buffer used in this method was 0.1% Perchloric acid buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 230nm. Retention time of Mupirocin and Fluticasone were found to be 2.146 min and 2.770 min. percentage relative standard deviation of the Mupirocin and Fluticasone were and found to be 0.4 and 0.5 respectively. Percentage Recovery was obtained as 98.75% and 99.42% for Mupirocin and Fluticasone respectively. Limit of detection, Limit of quantitation values obtained from regression equations of Mupirocin and Fluticasone were 0.38, 1.16 and 0.02, 0.05 respectively. Regression equation of Mupirocin is y = 10256.x + 82433, and y= 24529x + 3330 of Fluticasone. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries. Key Words: Mupirocin, Fluticasone, Quality control, Retention time, Limit of detection (LOD) and limit of quantification (LOQ), Regression equation.