EVALUATION OF α-GLUCOSIDASE, UREASE INHIBITION AND ANTIOXIDANT POTENTIAL OF ACACIA JACQUEMONTII AND RHAMNUS PERSICA

The aim of this study was to investigate antioxidant, urease and α-glucosidase inhibition activities of the plants Acacia jacquemontii and Rhamnus persica. Dichloromethane and methanol extracts of the plants were evaluated for described activities. In α-glucosidase inhibition assay, dichloromethane and methanolic extracts of Acacia jacquemontii exhibited inhibitory activity of 97.9 % and 98.9 % with IC50 of 4.8 μg/ml and 1.2 μg/ml respectively while that of Rhamnus persica showed 68.8 % and 24.5% with IC50 values of 29.3 and 614.5 μg/ml respectively. The results were compared with standard, Acarbose, which showed 70.1 % inhibition with IC50 of 520 μg/ml. DPPH inhibition assay indicated that both dichloromethane and methanolic extracts of Acacia jacquemontii were active with percentage inhibition of 86.8% and 94.11 % respectively with IC50 values of 24.51 μg/ml and 9.51 μg/ml. The dichloromethane and methanolic extract of Rhamnus persica exhibited the percentage inhibition of 75.9 and 94.9 respectively with IC50 values 30.95 μg/ml and 34.77 μg/ml. Ascorbic acid was used as standard. Both the plants showed non significant activity in urease inhibition assay. These results confirm that both plant extracts possess significant α-glucosidase inhibitors and antioxidant potential, thereby providing worthy justification for isolation of novel bioactive compounds. Further study is needed to screen out antioxidants and potent enzyme inhibitors. Keywords: Acacia jacquemontii, Rhamnus persica, Antioxidant, α-Glucosidase inhibition, Urease inhibition