DEVELOPMENT AND VALIDATION OF UV-SPECTROSCOPIC METHOD FOR THE ESTIMATION OF METFORMIN IN BULK AND PHARMACEUTICAL DOSAGE FORM

A new simple, accurate, rapid, precise, reproducible and cost effective spectrophotometric method for the quantitative estimation of Metformin in bulk and pharmaceutical dosage form. The developed visible spectrophotometric method for the quantitative estimation of Metformin is based on measurement of absorption at maximum wavelength 242 nm using Methanol as a solvent. The stock solution of Metformin was prepared, and subsequent suitable dilution was prepared with methanol to obtained standard curve. The standard solution of Metformin shows absorption maxima at 242 nm. The drug obeyed beer lambert's law in the concentration range of 0 - 60 μg/ml with regression 0.999 at 242 nm. The overall % recovery was found to be 100.04% which reflects that the method was free from the interference of the impurities and other excipients used in the bulk and marketed dosage form. The low value of % RSD was indicative of accuracy and reproducibility of the method. The % RSD for inter-day and intra-day precision was found to be 0.53 and 0.51respectively which is<2% hence proved that method is precise. The results of analysis have been validated as per International Conference on Harmonization (ICH) guidelines. The developed method can be adopted in routine analysis of Metformin in bulk and tablet dosage form. Keywords: Metformin, UV Visible Spectrophotometry, Method development, Validation, , Methanol , Accuracy, Precision.