DEVELOPMENT OF NEW RP-HPLC METHOD FOR SIMULTAENOUS ESTIMATION OF AMILORIDE AND HYDROCHLOROTHIAZIDE

A simple, Accurate, precise method was developed for the simultaneous estimation of the Amiloride and Hydrochlorthiazide in Tablet dosage form. Chromatogram was run through BDS (250mm 4.6mm, 5µ). Mobile phase containing Buffer and Acetonitrie in the ratio of 45:55A was pumped through column at a flow rate of 1ml/min. Temperature was maintained at 30°C. Optimized wavelength for Amiloride and Hydrochlorthiazide was 296nm. Retention time of Amiloride and Hydrochlorthiazide were found to be 4.081 min and 2.554 min. %RSD of the Amiloride and Hydrochlorthiazide were and found to be 0.57 and 0.71 respectively. %Recover was Obtained as 100.40% and 99.9% for Amiloride and Hydrochlorthiazide. LOD, LOQ values were obtained from regression equations of Amiloride and Hydrochlorthiazide were 0.06ppm, 0.14ppm and 0.18ppm, 0.41ppm respectively. Regression equation of Amiloride is y = 8186x + 1334, and of Hydrochlorthiazide is y = 3525x + 408.9. Key Words: Amiloride, Hydrochlorthiazide, RP-HPLC