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Journal: Indo American Journal of Pharmaceutical Sciences (IAJPS) (Vol.05, No. 05)

Publication Date:

Authors : ;

Page : 3617-3630

Keywords : Naproxen; Esomeprazole; RP-HPLC method;

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Estimation of Naproxen and Esomeprazole simultaneously in tablet dosage forms by RP-HPLC method. The analytical method was developed by studying different parameters. First of all, maximum absorbance was found to be at 332nm Naproxen for and 285nm for Esomeprazole. Common wavelength will be 272nm and the peaks purity was excellent. Injection volume was selected to be 20µl which gave a good peak area. The column used for study was Inertsil C18, ODS chosen good peak shape. Ambient temperature was found to be suitable for the nature of drug solution. The flow rate was fixed at 1.0ml/min because of good peak area, satisfactory retention time and good resolution. Different ratios of mobile phase were studied, mobile phase with ratio of 45:55 Methanol: Buffer was fixed due to good symmetrical peaks and for good resolution. So this mobile phase was used for the proposed study. The present recovery was found to be 98.0-101.50 was linear and precise over the same range. Both system and method precision was found to be accurate and well within range. Detection limit was found to be 0.25 Naproxen and 0.52 for Esomeprazole. Linearity study was, correlation coefficient and curve fitting was found to be. The analytical method was found linearity over the range of 20-80ppm of the target concentration for both the drugs. The analytical passed both robustness and ruggedness tests. On both cases, relative standard deviation was well satisfactory. Keywords: Naproxen, Esomeprazole, RP-HPLC method

Last modified: 2018-05-21 01:13:04