Effect of Different Carbon Sources on in Vitro Shoot Regeneration of Petunia Hybrid Hort. (Vilm)

The present study was undertaken in order to develop an efficient in vitro propagation protocol for Petunia hybrid using leaf as an explant. The effects of different concentrations of five different carbon sources i.e., sucrose, glucose, mannose, arabinose and maltose was investigated on shoot regeneration of Petunia hybrid. Shoot regeneration occurred on MS medium supplemented with sucrose, glucose and maltose. No growth was obtained on MS medium when mannose and arabinose were used as carbon source. Best shoot regeneration with maximum shoot length and maximum number of shoots was obtained on MS medium supplemented with 3% carbon within 11 days with 70% culture response. Shoot regeneration was also obtained on MS medium supplemented with glucose and maltose within 18 and 24 days with 50 and 30% culture response respectively. Assia Zahoor | Jasfeeda Qadir | Dr. Seema Singh"Effect of Different Carbon Sources on in Vitro Shoot Regeneration of Petunia Hybrid Hort. (Vilm)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-4 , June 2018, URL: http://www.ijtsrd.com/papers/ijtsrd15641.pdf http://www.ijtsrd.com/biological-science/botany/15641/effect-of-different-carbon-sources-on-in-vitro-shoot-regeneration-of-petunia-hybrid-hort-vilm/assia-zahoor