Comparison of different STR Typing for a Special Allele at D7S820 Locus by Using Ten Different STR Multiplex System| Biomed Grid

During a DNA database construction and population study in China, we observed a special genotype 10/10.1 at D7S820 locus which was misjudged by seven out of ten different STR multiplex systems. Allele typing results acquired by using ten diverse STR multiplex kits (PowerPlex® Fushion6C, PowerPlex® 21, Power Plex®24, ACGU® EX25, Applied Bio® HuaXiaBaijin, SureID® PanGlobal, GoldeneyeTM20A, GoldenEyeTM25A, AppliedBio®GlobalFiler, HuaDa ® YanHuagn) and then analyzed by GeneMapper® 1.4 software. In this paper, we conclude the reasons for the misjudgment of this allele as well as how to reduce these kinds of errors during STR analysis.