Optimization of Slight Halotolerant α-Amylase Producing Brevibacillus Borstelensis R1 in Various Media by Submerged Fermentation

Bacteria in marine habitat have modified structure of enzymes, ribosomes, and transport proteins which require high levels of potassium for stability and activity. In the present study, α-amylase producing Brevibacillus borstelensis R1 was isolated from marine waters of Bay of Bengal, Rusikonda, Visakhapatnam by applying different primary screening techniques. The secondary screening of α-amylase production of Brevibacillus borstelensis R1 was carried out at varying % of NaCl using ten different media. The sodium chloride response towards 1.0%, 2.0%, 3.0% and 4.0% was positive and the response was negative at 5.0% NaCl. B.borstelensis R1 was categorized under slight halophile which shows optimum growth at 2?5% NaCl. Alpha-amylase produced by B.borstelensis R1 have many applications in starch processing, desizing of textiles, paper sizing, detergent additive, bread improvement, ethanol production, sewage treatment, effluent treatment and other fermentation processes.