DEMINERALIZATION OF TOOTH ENAMEL SURFACE AT DIFFERENT IMMERSION TIMES OF SPORT DRINK AND BUFFERING EFFECT OF CASEIN DERIVATIVE AGAINST ENAMEL EROSION

Introduction: Sports drinks are becoming increasingly popular as well as being encouraged with regular exercise. These drinks are less than normal pH and found to be acidic.These acidic bevarages are thought to increase potential for dental erosion.Casein Phosphopeptide - Amorphous Calcium Phosphate CPP-ACP complex helps to release Calcium, Phosphate ions to form apatite crystals. In acidic conditions, these calcium, phosphate ions are released from crystal complex and facilitate to reduce the extent of demineralization. This concept forms the basis for the study. Objective: To estimate the pH values of Sport drink(Redbull) before and after immersion time. To estimate the loss of mineral content of the tooth exposed to sports drink with and without CPP-ACP. Material & Methods: Extracted premolar teeth of human dentition free of dental caries and hypocalcification were selected. Teeth were sectioned to obtain 3 enamel sections from each tooth to obtain 15 sections. Specimens were immersed in Sports drink(Redbull) Procedure (A) ,Sportsdrink (Redbull) +CPP-ACP Procedure (B) and Control Procedure (C) for 48 hours,24 hours,12 hours,6 hours,3 hours. pH Values before and after procedure were recorded and analysed. After completion of immersion time, Tooth specimens were sectioned using Hard tissue Microtome viewed for extent of demineralization under Polarized light microscope. Results: Specimens subjected to Sports drink(A),showed wider areas of enamel demineralization. One way analysis of variance (ANOVA) between the groups shows that F value is 4.977,df=2, at 5% level of significance. Sportsdrink+ CPP-ACP (B)(193+ 14) showed decreased amount of demineralisation when compared to Sportsdrink (A)(264+19). Conclusion: CPP-ACP has the ability to reduce demineralization caused by sports drinks. It facilitates Protective action of Loss of mineral content of teeth caused by Sports drink erosion.