Identification&Study on Antibiogram of Salmonella Spp. Isolated through Clot Culture

Aim: To compare the effectiveness of the Widal screening& clot culture methods for typhoid /paratyphoid fever diagnosis in Widal positive patients. Study design: Analysis involved a experimental prospective study. Place& duration of study: The study took place at the Department of Medical Microbiology, at RNT Medical College between March 2018 –Sept.2018. Methodology: A total of 306 samples from 143 male& 163 female aged 15-70yr. The isolation from samples& identification were carried out with clot culture methods, characterization was done using polyvalent sera. The rates of recovery of S. Paratyphi A, B, C and typhi D from clot culture of individuals positive at the various Widal antibody titres were evaluated. Result: Among the subjects positive for Widal titre of O& H variants, the isolation rate from clot culture 40 % at titre 1: 320, 14 % at titre 1: 160& 10 %, p value is 0.045 indicates that isolation of organism are positively associated with increase ratio of antibody titre. Keywords: typhoid fever, widal, clot culture, Salmonella sp.