Identification and Analysis of GeneExpression in Flies Brain: An Insilico ApproachJournal: International Journal of Science and Research (IJSR) (Vol.9, No. 12)
Publication Date: 2020-12-05
Authors : Arpita Parakh; Naveen Dhingra;
Page : 438-448
Keywords : Neurodegeneration; tauopathy; biomarker; differential Expression; biological pathways;
Background: Recent advances in unscrambling the epigenetic and genetic keystones of tauopathies have occurred but less development is seen in treatments and effects after treatments. It is a challenge to prognosticate enormously complex area of tauopathy and neurodegeneration by finding out the mystery behind the differential expression of genes and its network during the process of neurodegeneration. Objective: The functional development of neurons is based on regulation of genetic blueprint of brain and is a matter of extensive research to understand the role and differential expression of genes and regulators to identify the novel targets and biomarkers responsible for identification of neurodegenerative disorders occurring due to DNA Damage, aging and tauopathy. Hence highthroughput genome data is exploited to predict the expression and function of genes involved during the process of neurodegeneration in TAU mutated flies brain. Methodology: Here we worked on chain of software to find out the hidden genomic mystery of TAU mutated flies against the control flies by analyzing their next generation sequencing data and identifying important landmarks in genes which express differentially in TAU mutant flies. The study shows differential expression of many genes involved in epigenetic regulation which can be used as prognostic biomarker in neurodegenrative disorders and tauopathies including Alzheimer disease (AD). Results: Our study implicates the role of heterochromatic condensation leads to apoptotic action in tauopathologies. The genes which are involved in notch signaling, autophagy and protein serine/threonine kinase pathway are found to be differentially expressed which gives a clue of their causal role in disrupting cell cycle, DNA damage, heterochromatin condensation, and apoptosis indicating the dreadful future of neurotoxicity in an individual. Conclusion: We focused specifically on pathways, and the genes which are involved in regulation of these pathways, and also found thata large class of highly conserved, small, noncoding RNAs that are responsible for RNA interference and found out their different expression in TAU mediated flies.
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