Effect of Yellow-Split Chickpeas on Hepatosteatosis-Induced Serum Oxidative Damage in Rats

Hepatosteatosis represents a severe health hazard to humans due to its prevalence in last time. Therefore, the present study was designed to evaluate the effect of phosphatidylcholine (PC), C. arietinum-enriched PUFA (CAP), and its prepared phytosome form (CAPP) against non-alcoholic fatty liver disease (NAFLD) in rats. Forty-eight rats were randomly divided into eight experimental groups. Control groups including normal, PC, CAP, and CAPP. Normal group received normal diet and distilled water for 45 days. Other control groups received normal diet and administered distilled water for 15 days then administered 500 mg/kg b. wt of PC, CAP, and CAPP, respectively for another 30 days. NAFLD group, rats received a high fat diet (HFD) and injected tamoxafene (TAM) (200 mg/kg body weight) intraperitoneally for 15 consecutive days, then administered distilled water for another 30 days. NAFLD + PC, NAFLD + CAP, and NAFLD + CAPP groups received HFD and injected with tamoxafene for 15 days, then administered PC, CAP, and CAPP, respectively for an additional 30 consecutive days. The results revealed that NAFLD elicited oxidative stress which was evident due to the significant increase of s-LPO, and significant decrease of s-GSH, s-SOD, s-GPx, and s-GRD. However, treatment with PC/ CAP/ CAPP ameliorates the oxidative changes caused by NAFLD. CAPP showed the best regimen which may be due to its phytosome form that allows bioavailability of CAP.