Green Synthesis of Iron Nanoparticles from Moringa Oleifera Seeds

Cold and Soxhlet method of extraction was carried out using Moringa oleifera seeds with aqueous and ethanol as solvents. The qualitative and quantitative analysis was performed which contains phytochemicals like alkaloids, phenols, glycosides, flavonoids, steroids, terpenoids, carbohydrates, proteins and saponins. The nanoparticles were synthesized using ferric chloride at varying ratios and were characterized using UV spectroscopy, SEM + EDAX, XRD& FTIR. The UV Spectra peaks showed absorption at 200 300nm. The antibacterial activity was performed using E. coli, P. aeruginosa, B. subtilis, S. aureus& S. pyogenes and the antifungal activity using P. chrysogenum, C. albicans& Aspergillus niger. The maximum zone of inhibition against E. coli and C. albicans was measured as 27mm and 32mm. Other applications were performed such as anticoagulation& thrombolytic activity was absent, 86 % of antioxidant activity was seen in CEME (Cold Extract M. oleifera ethanol), total antidiabetic activity was higher in CEMW (Cold Extract M. oleifera water), CEME has shown 26.36 % of anti inflammatory activity, 71 % of water hardness was removed using CEMW extract, CEMW& CEME gave better results for larvicidal activity, 59.76 % of heavy metals were removed by CEMW and these were found to be non toxic to the normal cells using MTT cytotoxicity assay.