Interspecies Variability between Wrightiatomentosa and W. tinctoria as Assessed by RAPD Analysis

RAPD markers were used for detection of variability between the two species in the genus Wrightia i. e. W. tomentosa and W. tinctoria. A total of 188 amplified bands were scored from 29 random decamer primers out of which 96 (51.06 %) were found to be polymorphic. On comparing the amplification products of DNA from both the species, it was observed that 2 primers produced similar amplified products in both the species, whereas 27 primers showed highly reproducible polymorphic banding pattern between these species. Highest percentage of polymorphism was found with the primer OP 26 (85 %) whereas no polymorphism was found with primers OP 16 and 27. The band size ranged from 200 bp to 3000 bp.