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Estimation of Cortisone Acetate in Pharmaceutical Anti-inflammatory Drugs by HPLC-UV Technique

Journal: International Journal of Science and Research (IJSR) (Vol.6, No. 2)

Publication Date:

Authors : ; ;

Page : 1178-1183

Keywords : Cortisone acetate; Oral Tablets; formulation; and HPLC- UV System;

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The HPLC-UV System was used to assay of Cortisone acetate in Oral Tablets So, compare the bioavailability of two types of Cortisone acetate, commercial formulations and Cortisone acetate analar grade as a test formulation. Cortisone acetate concentrations were analyzed by HPLC-UV System at (=254 nm). The separation was achieved by using the Ion Pac Ercus C18 RP-Column, 5m, 4.6250 mm. The mobile phase consisted of water /acetonitrile (5545). The study of bioequivalence between the two Cortisone acetate formulations were assessed by calculating peaks height. The standard Cortisone acetate eluted at a flow rate of 1.0 ml/min. The method was found to belinear in the range (0.5 to 2.5) g/ml (n = 5) with R2 0.9991, also, the recoveries were range within 99-100 %. The detection limit of quantification (LLOQ) was 0.07909g/ml and lower limit of detection (LLOD) 0.02610 g/ml. showing average intra assay and inter-assay coefficients of RSD % about 0.522 %. The results of recoveries, RSD, and statistical parameters obtained in this study. The accurate, precise and sensitive refers to validation method for determination of Cortisone acetate in anti-inflammatory Pharmaceutical drugs.

Last modified: 2021-06-30 17:48:27