CFIA-Turbidimetric and Photometric Determination of Vitamin B2 using LEDs as a Source of Irradiation and Two Solar Cells as an Energy Transducer

A new, sensitive and simple method has been used for the determination of vitamin B2 (Riboflavin) in pure and pharmaceutical formulations by continuous flow injection analysis. The method is based on oxidation vitamin B2 by sodium periodate to release formic acid then coupled with 2, 4 dinitrophenylhydrazin in an aqueous medium to obtain a yellow precipitate complex, using homemadeAyah-6SX1-ST-2D solar cell CFIA. Optimum parameters have been studied to increase the sensitivity and limit of detection for this developed method. The linear range for the instrument response versus vitamin B2concentration was (0.05-5.5) mMol. L-1while the L. O. D was 1.615g/sample, the correlation coefficient ( r) was 0.9825while percentage linearity ( r2 %) was 96.52 %. RSD % for the repeatability (n=8) was less than 0.5 %for the determination of vitamin B2 at concentration (1, 4) mMol. L-1 respectively. The method was applied successfully for the determination of vitamin B2 in pharmaceutical preparation. A comparison was made between two methods newly proposed method and the reference claimed method UV-SP spectrophotometry at max=445 nm, of analysis using the standard addition method via the use paired t-test. It showed that there was no significant difference between the two methods proposed (developed) method and the reference claimed ( classical method) for determination of vitamin B2 at 95 % confidence level.