Antimicrobial Activity of Pseudomonas Spp under Different pH, Temperature and Nutritional Values

Antimicrobial activity is ability to destroy and inhibit the growth of other microorganisms. Effect of stress conditions on antimicrobial activity is variable. Antimicrobial activities of the isolates were checked against two species of Bacillus and two species of E. coli. The microbes exhibiting the antimicrobial activity can affect their host in different ways, this effect may be stimulatory or inhibitory under different environmental factors such as pH, temperature and minimal media. Cell morphology and gram staining behavior of these strains were also studied after different intervals, at different pH and temperature conditions both in broth and agar. It can be concluded that antimicrobial activity was manifested by the bacterial strains mostly at pH 7 and 37C on L-agar. On minimal media antimicrobial activity was not expressed. When different concentrations of glucose, fructose and peptone supplements were added to the minimal media antimicrobial activity was retained by the bacterial strains. Cell morphology sporulation, cell size and staining behavior was affected with higher temperatures and pH. In L-broth effects of stress were more pronounced than in L-agar. Overall environmental affects either physical or chemical have important role in manifesting and inhibiting antimicrobial activity. The microorganisms showed prominent results can be analyzed further in the field of agriculture.