Micropropagation of Hoya Kerrii (Valentine Hoya) Through Callus Induction for Long Term Conservation and Dissemination

Micropropagation is applied to multiply those species which are difficult to produce conventionally. The purpose of this study was to access in vitro propagation of Hoya kerrii, an important ornamental plant to explore its potential for micro-propagation. Microprogation of Hoya kerrii was initiated using leaf, petiole, root and inter-nodal segments of the selected plant as explants on MS medium containing 2, 4-D at 1, 2, 3, 4 and 5 mg/L for callus induction. Leaf segments initiated callus earlier than inter-node, petiole and root. A significant amount of callus was produced in MS medium with 5.0 mg/L 2, 4-D and MS medium with 1.0 mg/L 2, 4-D gave the poorest callus.