Bioethanol Production by A. Niger Produced Cellulase by Solid State Fermentation

Bioethanol is the most suitable renewable alternative to fossil fuel as its quality constituent match with diesel and petrol. the agro residues (sugarcane bagasse, potato peel and rice bran) were delignified by 2 % NaOH and subjected for production of cellulase by Aspergillus niger under solid state fermentation. The highest cellulase activity was attained with sugarcane bagasse at pH 4.5, temperature-300C and incubation time 96h. The resultant crude enzyme of 5.0 U/ml were applied for saccharification of all pre-treated substrates in 0.1M citrate buffer (pH 4.8) and obtained maximum reducing sugars from sugarcane bagasse at pH 7.0, 400C temperature and incubation time 120h. Hydrolysates thus obtained were used for ethanol production by inoculating Saccharomyces cerevisiae under different fermentation conditions and observed maximum production with sugarcane bagasse at pH 5.5 after 48 h at 350C.