Identification of SSR Markers for Testing of Hybridity and Seed Genetic Purity in Maize (Zea Mays L.)Journal: International Journal of Science and Research (IJSR) (Vol.3, No. 10)
Publication Date: 2014-10-05
Authors : Sudharani M; Rao PS; Subba Rao LV;
Page : 92-98
Keywords : Genetic purity; Maize hybrids; SSR markers;
The present investigation was undertaken to identify specific marker associated with the maize hybrid DMH-117 and its parental lines, hybrid purity testing as well as identify the off-types from the seed lot. The hybrid DHM-117 could be clearly identified by using the SSR loci Umc1600 based on the banding pattern resolved on agarose gel (3 %). The complementary banding pattern of the male and female parents made a way to identify the hybrid. The SSR loci Umc1600 amplified a specific allele size of 180bp in seed parent (BML-6) and 195bp was specific to pollen parent BML-7. These two bands of allele size 180bp and 195bp were found in hybrid DHM-117, there by confirming that this hybrid was produced from the cross combination of BML-6 and BML-7.
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