Optimization of Growth, Fibrinolytic Enzyme Production and PCR Amplification of Encoding Fibrinolytic Enzyme Gene in Bacillus amyloliquefaciens Isolated from Ntoba mbodi at Brazzaville

Two strains of Bacillus amyloliquefaciens screened from Ntoba Mbodi, respectively B. a NM76 and B. a NM 77 were tested as strong producers of a fibrinolytic enzyme. Monitoring of growth and enzyme production were assayed. Experiments were conducted about the influence of temperature and PH on the growth and fibrinolytic enzyme production. For Temperature, growth and enzyme production were observed: from 25C to 50C with the optimum temperature between 35C to 40C for growth, and the optimum between 30C and 35C for fibrinolytic enzyme production. For pH from 5 to 11, growth was possible with the optimum at pH 7, enzyme production was possible in the range of pH from 5 to 9, with the optimum at 8. The relationship between growth and enzyme production has shown that the later usually is optimal during the stationary phase of the growth. Moreover, PCR amplification of the encoding fibrinolytic enzymes genes in the two strains has been hold and the results has shown two identical specific bands, as illustrated by the agarose gel electrophoresis of the PCR products.