Assessment of Lysozyme Activity in Bacteria Challenged Haemolymph of Achaea janata Larvae

Lysozyme was the first humoral antibacterial factor to be studied in insects and it was considered the main immune factor in the haemolymph. We have been investigating lysozyme activity in bacterial challenged haemolymph of Achaea janata larvae. This is a major crop pest and polyphagus lepidoptera insect. Results showed that the initially no activity after 5 min lysozyme activity was gradually increase and it reached maximum at 15 min post bacterial challenge haemolymph. Later lysozyme activity was constantly decreased. The present study we can conclude that Lysozyme shows its efficiency if not immediately but within 5-15 or 20 min time interval thus protecting the host cell and it may triggers the insect immune humoral response by lysis the lysozyme by the action of M. luteus bacteria challenge.