Changes in Protein Profile of Bacterial-Challenged Culex Pipiens Larvae

The present work aims to study the changes in protein profile of bacterial-challenged fourth instar Culex pipiens larvae. For achievement this research, fourth larval instars of Culex pipiens were challenged with Gram (+) bacteria, Staphylococcus aureus and gram (-) bacteria, Klebsiella pneumoniae and mix of the two bacterial strains to study the effect of bacteria on Culex pipiens larvae protein profile quantitative and qualitative at 6, 12, 18, 24, 30, 36, 42, 48, 54, 60, 66 and 72 hours postinfection (h. p. i. ). The results showed a significant increase of total protein of the bacterial-challenged fourth instar larvae and then declined over time. Significant gradual increases in total soluble protein concentration were observed within each treatment group at all tested times. Such increases reached plateau phase at 36 h. p. i. Significant increase in total soluble protein was observed in the case of treated larvae as compared with controls. Whole body proteins of control and bacterial-challenged fourth instar larvae were separated using native and SDS-PAGE at different times postinfection. The results of native-PAGE demonstrated that there were differences in the overall protein banding pattern in the infected larvae as compared to control. The results of the SDS-PAGE clarified that the molecular weight of the separated proteins ranged from 140 to 14 KDa and bacterial infection had led to the induction of different proteins as compared to control.