Kamdhenu Ark as a Genoprotective Agent Against Chlorpyrifos Induced Genotoxicit

Pesticides are being purposely added to the environment to control domestic and agricultural pests for increasing the yield of crops to satisfy the growing demands of population. The present study was aimed to evaluate the ameliorative effect of cow urine on chlorpyrifos-induced genotoxcity in vivo. Male healthy rats, 810 weeks old, weighing 120 10 g were randomly selected and divided into eight groups, namely, corn oil (C) ; cow urine (CU), Group P-1/8 of LD50 i. e 19mg/kg b. wt CPF, Group Q-1/4 of LD50 i. e 38mg/kg b. wt CPF, Group R-1/2 of LD50 i. e 76mg/kg b. wt CPF, Group X-1/8 of LD50 i. e 76mg/kg b. wt + cow urine, Group Y-1/4 of LD50 i. e 38mg/kg b. wt + cow urine, Group Z-1/2 of LD50 i. e 19mg/kg b. wt + cow urine. All treatments were administered orally for 24, 48, 72 hours. Chlorpyrifos treated group showed increased chromosomal aberrations, as compared to controls. The groups pretreated with cow urine exhibited a significant decrease in frequency of aberrant cells as compare to the rats treated with chlorpyrifos alone. The present findings clearly show that oral administration of cow urine protects the rats from chlorpyrifos induced DNA damage and suggests that this treatment alleviates the genotoxicity of chlorpyrifos to a greater extent.